6 protocols
AccessionNameType
P-GSE52507-1
normalization data transformation protocol
GeneSpring GX (v. 7.3.1) was used for background subtraction and LOWESS normalization. ID_REF = VALUE = Lowess-normalized log2 ratio of expression (small fish/large fish) PRE_VALUE = Lowess-normalized ratio of expression (small fish/large fish)
P-GSE52507-6
array scanning protocol
Images were scanned on a GenePix 4200A scanner. Images were quantified using Genepix Pro software (version 6.1).
P-GSE52507-5
hybridization protocol
cDNA was hybridized according to Preventing Ozone Related Problems version of the the hybridization protocol described in Two-Color Microarray-Based Gene Expression Analysis (Agilent Technologies) in Agilent hybridization chambers.
P-GSE52507-4
labelling protocol
cDNA was prepared from 0.5 ug mRNA using a SuperScript® Plus Direct cDNA Labeling System with Alexa Fluor® aha-dUTPs and labelled with either Alexa Fluor 555 or Alexa Fluor 647 according to the manufacturer's instructions.
P-GSE52507-3
nucleic acid extraction protocol
Total RNA extracted using Trizol following manufacturer's instructions. mRNA was extracted from total RNA using μMACS mRNA Isolation Kits – Small Scale according to the manufacturer's instructions.
P-GSE52507-2
growth protocol
Rainbow trout from the Lyndon strain were reared at Alma Aquaculture Research Station and fed ad libitum.