E-GEOD-5239 - Transcription profiling of E. coli BL21, BL21/pOri1 and BL21/pOri2mutants

Status
Submitted on 6 July 2006, released on 13 June 2008, last updated on 10 June 2011
Organism
Escherichia coli
Samples (24)
Array (1)
Protocols (4)
Description
Transcription profiles in BL21, BL21/pOri1 and BL21/pOri2 were analysed using DNA microarray technology. BL21, BL21/pOri1 or BL21/pOri2 strains were cultured at chemostat status and harvested after the cultivation arrived steady status. Experiment Overall Design: BL21, BL21/pOri1 and BL21/pOri2 were cultured at the exponential growth status or at the same growth rate status, respectively. E. coli BL21, BL21/pOri1 and BL21/pOri2 RNAs were reverse-transcripted into cDNAs, The RNAs from the BL21, BL21/pOri1, BL21/pOri2 were labelled with biotin. The labelled cDNAs were mixed and then hybridized on the microarray slides. Experiments were repeated four times. Most of them have no significant difference comparing plasmid-carrying E. coli and plasmid-free E. coli
Experiment types
transcription profiling by array, unknown experiment type
Contact
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-5239.idf.txt
Sample and data relationshipE-GEOD-5239.sdrf.txt
Processed data (1)E-GEOD-5239.processed.1.zip
Array designA-AFFY-29.adf.txt
Links