E-GEOD-5232 - Transcription profiling of Lbeta T2 cells treated with either vehicle or activin A for different length of time to characterize the upregulation of gene expression by activin A.
Submitted on 5 July 2006, released on 27 November 2007, last updated on 10 June 2011
The primary response of Lbeta T 2cells upon activin treatment at 1hr, 2hr, and 6hr is the significant up-regulation of FSHbeta subunit. Affymetrix genearray missed this important information due to cross-hybridization of its probes with other genes and chromosome segments. Activin modulates a network of genes and signaling pathways to exert a variety of functions. Experiment Overall Design: Subconfluent Lbeta T2 cells were seeded in cell culture flasks for 16 hours in phenol-free, serum-free medium, and then treated with either vehicle or activin A (30ng/ml). At 1hr, 2hr, or 6hr after treatment, total RNA was isolated using RNeasy Mini Kit. Integrity of RNA was evaluated with an Agilent 2100 Bioanalyzer, and purity/concentration was determined using a GeneSpec III before target preparationi and hybridizations.
transcription profiling by array, co-expression, compound treatment, time series