E-GEOD-5229 - Transcription profiling of human and mouse keratinocytes infected with AD and Ad-NotchIC viruses reveals cross-regulation between Notch and p63 in keratinocyte commitment to differentiation

Status
Submitted on 5 July 2006, released on 13 June 2008, last updated on 27 March 2012
Organism
Homo sapiens, Mus musculus
Samples (4)
Arrays (2)
Description
Notch signaling promotes commitment of keratinocytes to differentiation and suppresses tumorigenesis. p63, a p53 family member, has been implicated in establishment of the keratinocyte cell fate and/or maintenance of epithelial self-renewal. Here we show that p63 expression is suppressed by Notch1 activation in both mouse and human keratinocytes through a mechanism independent of cell cycle withdrawal and requiring down-modulation of selected interferon-responsive genes, including IRF7 and/or IRF3. In turn, elevated p63 expression counteracts the ability of Notch1 to restrict growth and promote differentiation. p63 functions as a selective modulator of Notch1-dependent transcription and function, with the Hes-1 gene as one of its direct negative targets. Thus, a complex cross-talk between Notch and p63 is involved in the balance between keratinocyte self-renewal and differentiation. Experiment Overall Design: PolyA+ mRNA (2-5 ug) from cells infected with the Ad GFP and Ad-NotchIC viruses was used as template for double stranded cDNA preparations with a T7-(dT)24 oligonucleotide primers for the first strand reaction. The resulting cDNA was used for preparation of biotin-labeled antisense cRNA and further used for hybridization to Affymetrix U74A or U95A “gene chips” according to the manufacturer’s recommendation.
Experiment types
transcription profiling by array, unknown experiment type
Contact
Citation
Cross-regulation between Notch and p63 in keratinocyte commitment to differentiation. Bach-Cuc Nguyen, Karine Lefort, Anna Mandinova, Dario Antonini, Vikram Devgan, Giusy Della Gatta, Maranke I Koster, Zhuo Zhang, Jian Wang, Alice Tommasi di Vignano, Jan Kitajewski, Giovanna Chiorino, Dennis R Roop, Caterina Missero, G Paolo Dotto. Genes Dev 20(8):1028-42 (2006)
MIAME
PlatformsProtocolsFactorsProcessedRaw
Files
Investigation descriptionE-GEOD-5229.idf.txt
Sample and data relationshipE-GEOD-5229.sdrf.txt
Processed data (1)E-GEOD-5229.processed.1.zip
Array designsA-AFFY-36.adf.txt, A-AFFY-37.adf.txt
Links