E-GEOD-52104 - Gene expression profiles of stromal cells in ovarian cancer co-culture models

Status
Released on 1 December 2013, last updated on 3 June 2014
Organism
Homo sapiens
Samples (18)
Array (1)
Protocols (5)
Description
Goal: to profile gene expression change that occur when cancer-associated firboblast precursor cells are co-cultured with normal epithelial cells (IOSE4) or epithelial ovarian cancer cells (HeyA8) MSCs and INOF cells were co-cultured with IOSE4 cells or Hey.A8 EOC cells. 1000 stromal cells were plated into 6 well plates and 10,000 epithelial cells plated into porous inserts (pore size 1μm, Greiner BioOne). After 24hrs, cells were refed and epithelial cell inserts added to the stromal cell cultures. Cells were grown in 50% INOF medium and 50% MSC medium for 7 days before harvesting RNA
Experiment type
transcription profiling by array 
Contacts
Kate Lawrenson <kate.lawrenson@med.usc.edu>, Joshua Millstein, Simon A Gayther
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-52104.idf.txt
Sample and data relationshipE-GEOD-52104.sdrf.txt
Additional data (1)E-GEOD-52104.additional.1.zip
Array designA-MEXP-1171.adf.txt
Links