E-GEOD-5187 - Transcription profiling of Arabidopsis nst1-1 nst3-1 mutants
Submitted on 28 June 2006, released on 13 June 2008, last updated on 3 May 2014
Wood is one of the most important and enormous biomass that is widely used in our life. It is formed by successive addition of secondary xylem that develops continuously from cambium. The transcriptome of nst1 nst3 “double-knockout” lines was examined to know the effect of mutations on wood formation. Experiment Overall Design: Total RNA was extracted from the base 4 cm part of inflorescence stems whose heights were between 12 and 17 cm of three independent nst1-1 nst3-1 double T-DNA tagged lines and the whole transcriptome was compared with that of wild-type plant.
transcription profiling by array, unknown experiment type
NAC transcription factors, NST1 and NST3, are key regulators of the formation of secondary walls in woody tissues of Arabidopsis. Nobutaka Mitsuda, Akira Iwase, Hiroyuki Yamamoto, Masato Yoshida, Motoaki Seki, Kazuo Shinozaki, Masaru Ohme-Takagi. Plant Cell 19(1):270-80 (2007)