E-GEOD-51861 - RNA-seq (Illumina and PacBio) of hESC

Status
Released on 30 October 2013, last updated on 25 November 2013
Organism
Homo sapiens
Samples (2)
Protocols (4)
Description
We used PacBio data to identify more reliable transcripts from hESC, based on which we can estimate gene/transcript abundance better from Illumina data. PacBio long reads and Illumina short reads were generated from the same hESC cell line H1. PacBio reads were error-corrected by Illumina reads to identify transcripts. rSeq is used to estimate gene/transcript abundance of the identified transcriptome.
Experiment type
RNA-seq of coding RNA 
Contacts
Kin Fai Au <kinfai@stanford.edu>, Brian A Williams, Eric Schadt, Honoratus Van Bakel, Jason Underwood, Kin-Fai Au, Lawrence Lee, Vittorio Sebastiano, Wing H Wong
MINSEQE
Exp. designProtocolsFactorsProcessedSeq. reads
Files
Links