E-GEOD-5185 - Transcription profiling of S. cerevisiae Spt15p mutant and selection led to dominant mutations that conferred increased tolerance and more efficient glucose conversion to ethanol

Status
Submitted on 28 June 2006, released on 13 June 2008, last updated on 10 June 2011
Organism
Saccharomyces cerevisiae
Samples (12)
Array (1)
Protocols (4)
Description
Global transcription machinery engineering (gTME) is an approach for reprogramming gene transcription to elicit cellular phenotypes important for technological applications. Here we show the application of gTME to Saccharomyces cerevisiae for improved glucose/ethanol tolerance, a key trait for many biofuels programs. Mutagenesis of the transcription factor Spt15p and selection led to dominant mutations that conferred increased tolerance and more efficient glucose conversion to ethanol. The desired phenotype results from the combined effect of three separate mutations in the SPT15 gene [serine substituted for phenylalanine (Phe177Ser) and, similarly, Tyr195His, and Lys218Arg]. Thus, gTME can provide a route to complex phenotypes that are not readily accessible by traditional methods. Experiment Overall Design: We measured transcription levels for two strains (wild type control and mutant spt15) under normal (0% ethanol, 20 g/L glucose) and stress (5% ethanol, 60 g/L glucose) in biological triplicate.
Experiment types
transcription profiling by array, unknown experiment type
Contact
MIAME
PlatformsProtocolsFactorsProcessedRaw
Files
Investigation descriptionE-GEOD-5185.idf.txt
Sample and data relationshipE-GEOD-5185.sdrf.txt
Raw data (1)E-GEOD-5185.raw.1.zip
Processed data (1)E-GEOD-5185.processed.1.zip
Array designA-AFFY-47.adf.txt
R ExpressionSetE-GEOD-5185.eSet.r
Links