E-GEOD-51639 - E. coli rnpb mutants: wild type proxy strain DW2/pFLP-Ec vs. DW2/pFLP-Bs strain

Status
Released on 25 October 2013, last updated on 3 May 2014
Organism
Escherichia coli
Samples (10)
Array (1)
Protocols (7)
Description
Transcriptional profiling of DW2 E. coli cells in exponential growth phase that have a chromosomal deletion of the rnpb gene (which encodes the catalytic subunit of Ribonuclease P). We compared the test strain DW2/pFLP-Bs that expresses Bacillus subtilis rnpb from plasmid pFLP-Bs to reference strain DW2/pFLP-Ec, which expresses E. coli rnpb from plasmid pFLP-Ec. Two-strain experiment, wildtype proxy strain DW2/pFLP-Ec (reference) vs DW2/pFLP-Bs RT-10-2 (test) on each array. Biological replicates: 1 reference, 2 test. Four slides plus one dyeflip slide
Experiment type
transcription profiling by array 
Contacts
Jasmine L. Loveland <geo@ncbi.nlm.nih.gov>, Jasmine L Loveland, Robert Dorit
MIAME
PlatformsProtocolsFactorsProcessedRaw
Files
Investigation descriptionE-GEOD-51639.idf.txt
Sample and data relationshipE-GEOD-51639.sdrf.txt
Raw data (1)E-GEOD-51639.raw.1.zip
Processed data (1)E-GEOD-51639.processed.1.zip
Array designA-GEOD-17833.adf.txt
Links