E-GEOD-51604 - Memory B cell subset defined by CD80 and PD-L2 surface expression

Status
Released on 6 May 2014, last updated on 3 June 2014
Organism
Mus musculus
Samples (12)
Array (1)
Protocols (5)
Description
NP-reactive murine splenic memory B cells were sorted based on the expression of the surface markers CD80 and PD-L2 AM14 tg+ X Vk8R+/- recipients of B1-8+ B cells were immunized with NP-CGG and 8 weeks later splenic EMA-, CD19+ and NP+ memory B cells were sorted based on their surface expression of CD80 and PD-L2 (DN=CD80- PDL2-, SP=CD80- PDL2+, DP=CD80+ PDL2+). 1x10E5 up to 3x10E5 cells per sample were sorted and total RNA was isolated using the RNeasy plus micro kit and cRNA was prepared using the Illumina TotalPrep RNA Amplification Kit.
Experiment type
transcription profiling by array 
Contacts
Hailong Meng <geo@ncbi.nlm.nih.gov>, Florian Weisel, Mark Shlomchik, Steven Kleinstein
MIAME
PlatformsProtocolsFactorsProcessedRaw
Files
Investigation descriptionE-GEOD-51604.idf.txt
Sample and data relationshipE-GEOD-51604.sdrf.txt
Processed data (1)E-GEOD-51604.processed.1.zip
Array designA-MEXP-1175.adf.txt
Links