E-GEOD-51563 - Gene expression profile of a S. cerevisiae strain deleted in CSN5/RRI1 gene (systematic name YDL216C)

Status
Released on 23 October 2013, last updated on 3 May 2014
Organism
Saccharomyces cerevisiae
Samples (4)
Array (1)
Protocols (6)
Description
The COP9 signalosome (CSN) is a highly conserved eukaryotic protein complex which regulates the Cullin-RING family of ubiquitin ligases and carries out a deneddylase activity that resides in subunit 5 (CSN5). The budding yeast CSN is biochemically active and deletion mutants of each of its subunits exhibit deficiency in deneddylation of cullins, although the biological context of this activity is still unknown in this organism. To further characterize CSN function in budding yeast, we present here a transcriptomic analysis of a S. cerevisiae strain deleted in CSN5/RRI1 gene (hereafter referred to as CSN5), coding for the only canonical subunit of the complex. We show that Csn5 is involved in the modulation of the genes controlling aminoacid and lipid metabolism, and especially ergosterol biosynthesis. These alterations in gene expression correlate with the lower ergosterol levels and increased intracellular zinc content which we observed in csn5 null mutant cells. Two biological replicates, csn5 deleted strain vs. isogenic wild-type strain W303
Experiment type
transcription profiling by array 
Contacts
Valerio Licursi <valerio.licursi@uniroma1.it>, Chiara Salvi, Rodolfo Negri, Virginia De Cesare
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-51563.idf.txt
Sample and data relationshipE-GEOD-51563.sdrf.txt
Raw data (1)E-GEOD-51563.raw.1.zip
Processed data (1)E-GEOD-51563.processed.1.zip
Array designA-GEOD-14755.adf.txt
Links