E-GEOD-51525 - Transcriptomic analysis of chlorhexidine shock in Acinetobacter baumannii

Status
Released on 3 December 2013, last updated on 3 May 2014
Organism
Acinetobacter baumannii
Samples (8)
Array (1)
Protocols (6)
Description
In this study the transcriptomic changes occurring in A. baumannii ATCC 17978 in response to shock treament using the biocide chlorhexdine were determined. A. baumannii ATCC 17978 cells were grown at 37°C with shaking (200rpm) in 35mL cultures in MH broth to OD600=0.75, at which time they were split into 15mL cultures. One 15mL sample was treated with 4µg/mL chlorhexidine (0.5x MIC), whereas the other was not treated and used as a reference. Cultures were allowed to grow for a further 30 mins (to an average final OD600= ~1.35 for untreated cells and 1.25 for chlorhexidine treated samples), when cells were harvested by centrifugation and immediately suspended in Trizol reagent (Invitrogen). Total RNA was extracted using the PureLinkTM Micro-to-Midi Total RNA Purification kit (Invitrogen), incorporating an on-column DNAseI (Invitrogen) digestion. The experiment included using four grids of a custom Agilent 8 x 15000 spot microarray. Three biological replicates and one flip dye experiment.
Experiment type
transcription profiling by array 
Contacts
Karl Adam Hassan <karl.hassan@mq.edu.au>, Ian T Paulsen, Karl A Hassan
Citation
Transcriptomic and biochemical analyses identify a family of chlorhexidine efflux proteins. Hassan KA, Jackson SM, Penesyan A, Patching SG, Tetu SG, Eijkelkamp BA, Brown MH, Henderson PJ, Paulsen IT. , Europe PMC 24277845
MIAME
PlatformsProtocolsFactorsProcessedRaw
Files
Investigation descriptionE-GEOD-51525.idf.txt
Sample and data relationshipE-GEOD-51525.sdrf.txt
Raw data (1)E-GEOD-51525.raw.1.zip
Processed data (1)E-GEOD-51525.processed.1.zip
Array designA-GEOD-17813.adf.txt
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