E-GEOD-5150 - Transcription profiling of human hematopoietic stem/progenitor cells treated with TGF-beta1 to idetntify its target genes

Status
Submitted on 26 June 2006, released on 13 June 2008, last updated on 10 June 2011
Organism
Homo sapiens
Samples (4)
Array (1)
Protocols (12)
Description
CD34+ hematopoietic stem/progenitor cells were isolated from human cord blood and amplified in vitro for 10-14 days in serum-free medium with specific cytokines (Ju et al., Eur. J. Cell Biol. 82, 75-86, 2003; Hacker et al., Nat. Immunol. 4, 380-386, 2003). Cells were then treated with TGF-beta1 for various periods of time (2, 4, 16 hours) and RNA was prepared and subjected to microarray analysis. Experiment Overall Design: CD34+ hematopoietic stem/progenitor cells (HPC) were amplified in vitro and treated with TGF-beta1 (10 ng/ml) for 2, 4 and 16 hours. Experiment Overall Design: HPC untreated Experiment Overall Design: HPC + TGF-beta1 for 2 hours Experiment Overall Design: HPC + TGF-beta1 for 4 hours Experiment Overall Design: HPC + TGF-beta1 for 16 hours
Experiment types
transcription profiling by array, unknown experiment type
Contact
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-5150.idf.txt
Sample and data relationshipE-GEOD-5150.sdrf.txt
Raw data (1)E-GEOD-5150.raw.1.zip
Processed data (1)E-GEOD-5150.processed.1.zip
Array designA-AFFY-1.adf.txt
R ExpressionSetE-GEOD-5150.eSet.r
Links