E-GEOD-5147 - Transcription profiling of control and heat resistant Drosophila adult females before and at 8 time points after heat stress

Status
Released on 26 June 2007, last updated on 21 February 2012
Organism
Drosophila melanogaster
Samples (36)
Array (1)
Description
Gene expression was measured in control and heat resistance selected adult female flies before and at 8 time points after heat stress for 1h @ 36 degrees,Abstract The availability of full genome sequences has allowed the construction of microarrays, with which screening,of the full genome for changes in gene expression is possible. This method can provide a wealth of information about,biology at the level of gene expression and is a powerful method to identify genes and pathways involved in various,processes. In this study, we report a detailed analysis of the full heat stress response in Drosophila melanogaster,females, using whole genome gene expression arrays (Affymetrix Inc, Santa Clara, CA, USA). The study focuses on,up- as well as downregulation of genes from just before and at 8 time points after an application of short heat hardening,(368C for 1 hour). The expression changes were followed up to 64 hours after the heat stress, using 4 biological,replicates. This study describes in detail the dramatic change in gene expression over time induced by a short-term,heat treatment. We found both known stress responding genes and new candidate genes, and processes to be involved,in the stress response. We identified 3 main groups of stress responsive genes that were early–upregulated, early–,downregulated, and late–upregulated, respectively, among 1222 differentially expressed genes in the data set. Comparisons,with stress sensitive genes identified by studies of responses to other types of stress allow the discussion of,heat-specific and general stress responses in Drosophila. Several unexpected features were revealed by this analysis,,which suggests that novel pathways and mechanisms are involved in the responses to heat stress and to stress in,general. The majority of stress responsive genes identified in this and other studies were downregulated, and the,degree of overlap among downregulated genes was relatively high, whereas genes responding by upregulation to heat,and other stress factors were more specific to the stress applied or to the conditions of the particular study. As an,expected exception, heat shock genes were generally found to be upregulated by stress in general.
Experiment types
 co-expression, stimulus or stress, time series, transcription profiling by array
Contact
Citation
Full genome gene expression analysis of the heat stress response in Drosophila melanogaster. Jesper G Sørensen, Morten M Nielsen, Mogens Kruhøffer, Just Justesen, Volker Loeschcke.
MIAME
PlatformsProtocolsFactorsProcessedRaw
Files
Investigation descriptionE-GEOD-5147.idf.txt
Sample and data relationshipE-GEOD-5147.sdrf.txt
Processed data (1)E-GEOD-5147.processed.1.zip
Array designA-AFFY-17.adf.txt
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