E-GEOD-51466 - Polarization profiling of renal macrophages in stone model mice

Status
Released on 22 October 2013, last updated on 3 June 2014
Organism
Mus musculus
Samples (12)
Array (1)
Protocols (6)
Description
We recently reported elimination of renal crystals and migration of macrophages (Mφ) around crystals in hyperoxaluric mice, suggesting Mφs might eliminate crystals. Mφs are of 2 phenotypes: inflammatory (M1) and anti-inflammatory (M2). Because M2Mφs are considered to be involved in tissue repair and regeneration, we focused attention on their suppressive role in renal crystal formation. Hence, we investigated the gene array profiling of renal macrophages in stone model mice and CSF-1-deficient stone model mice. B6C3Fe a/a-Csf1op/J wild type/homozygous mice were admistered 80mg/kg gyyoxylate (GOX) for 6days, then the kidneys were extracted and sorted CD11b+/CD11c+ macrophages using autoMACS. The gene expression of WT- and op/op-macrophages were investigated using bone marrow derived M1/M2 macrophages as controls.
Experiment type
transcription profiling by array 
Contacts
MIAME
PlatformsProtocolsFactorsProcessedRaw
Files
Investigation descriptionE-GEOD-51466.idf.txt
Sample and data relationshipE-GEOD-51466.sdrf.txt
Raw data (1)E-GEOD-51466.raw.1.zip
Processed data (1)E-GEOD-51466.processed.1.zip
Array designA-GEOD-10787.adf.txt
Links