E-GEOD-5146 - Transcription profiling of Drosophila femalse selected for heat resistance

Status
Submitted on 23 June 2006, released on 13 June 2008, last updated on 10 June 2011
Organism
Drosophila melanogaster
Samples (10)
Array (1)
Description
gene expression was measured in five independent heat resistance selected replicate lines and five control lines. The genetic architecture underlying heat resistance remains partly unclear despite the well documented involvement of heat shock proteins (Hsps). It was previously shown that factors besides Hsps are likely to play an important role for heat resistance. In this study, gene expression arrays were used to make replicate measurements of gene expression before and up to 64h after a mild heat stress treatment, in flies selected for heat resistance and unselected control flies, to identify genes differentially expressed in heat resistance selected flies. We found 108 genes upregulated and 10 downregulated using the Affymetrix gene expression platform. Among the upregulated genes, a substantial number are involved in the phototransduction process. Another group of genes upregulated in selected flies is characterized by also responding to heat shock treatment several hours after peak induction of known Hsps revert to non-stress levels. These findings suggest phototransduction genes to be critically involved in heat resistance, and support a role for components of the phototransduction process in stress sensing mechanisms. In addition the results suggest yet uncharacterized genes responding to heat stress several hours after treatment to be involved in heat stress resistance. These findings mark an important increase in the understanding of heat resistance. see:; Cell Stress and Chaperones, vol. 11, Issue 4 (2006), pp 325-334,; DOI: 10.1379/CSC-207.1 Experiment Overall Design: gene expression was measured in five independent heat resistance selected replicate lines and five control lines
Experiment types
transcription profiling by array, unknown experiment type
Contact
MIAME
PlatformsProtocolsFactorsProcessedRaw
Files
Investigation descriptionE-GEOD-5146.idf.txt
Sample and data relationshipE-GEOD-5146.sdrf.txt
Processed data (1)E-GEOD-5146.processed.1.zip
Array designA-AFFY-17.adf.txt
Links