E-GEOD-5144 - Transcription profiling of human CD8 T lymphocytes from two healthy donors transduced with hTERT

Status
Released on 13 June 2008, last updated on 27 March 2012
Organism
Homo sapiens
Samples (20)
Arrays (2)
Protocols (5)
Description
Expression in GFP vs. GFP/hTERT transduced CD8 T Lymphocytes from Healty Donors (HD) 1 and 2 at early and late passages. Using CD8+ T lymphocyte clones over-expressing telomerase weinvestigated the molecular mechanisms that regulate T cell proliferation. Transduction and subcloning procedures were performed on CD8 + naive T-cell clones isolated from two different healthy individuals aged between 30 to 35 years (HD1 and HD2). T-cell cloneswere transduced to express hTERT/GFP or GFP alone. Experiment Overall Design: triplicates of 4 conditions for each of 2 donors, 1 chip (hTERT_YOUNG_HD1_REP2) of the first donor excluded because of low quality hybridization, one condition (GFP YOUNG)lost from second donor because of problems in cell culture.
Experiment types
transcription profiling by array, unknown experiment type
Contact
Citation
Mechanisms regulating the proliferative potential of human CD8+ T lymphocytes overexpressing telomerase. Olivier Menzel, Marco Migliaccio, Darlene R Goldstein, Sophie Dahoun, Mauro Delorenzi, Nathalie Rufer. J Immunol 177(6):3657-68 (2006)
MIAME
PlatformsProtocolsFactorsProcessedRaw
Files
Investigation descriptionE-GEOD-5144.idf.txt
Sample and data relationshipE-GEOD-5144.sdrf.txt
Processed data (1)E-GEOD-5144.processed.1.zip
Array designsA-AFFY-33.adf.txt, A-AFFY-44.adf.txt
Links