E-GEOD-5143 - Transcription profiling of human CD* T lymphocytes overexpressing hTERT

Status
Submitted on 23 June 2006, released on 13 June 2008, last updated on 27 March 2012
Organism
Homo sapiens
Samples (9)
Array (1)
Protocols (4)
Description
Using CD8+ T lymphocyte clones over-expressing telomerase weinvestigated the molecular mechanisms that regulate T cell proliferation. Transduction and subcloning procedures were performed on CD8 + naive T-cell clones isolated from two different healthy individuals aged between 30 to 35 years (HD1 and HD2). T-cell cloneswere transduced to express hTERT/GFP or GFP alone. HD1 was profiled on U133A and submitted as a separate GEO series. Experiment Overall Design: triplicates of 4 conditions, 1 condition (GFP YOUNG) lost because of problems in cell culture.
Experiment types
transcription profiling by array, unknown experiment type
Contact
Citation
Mechanisms regulating the proliferative potential of human CD8+ T lymphocytes overexpressing telomerase. Olivier Menzel, Marco Migliaccio, Darlene R Goldstein, Sophie Dahoun, Mauro Delorenzi, Nathalie Rufer. J Immunol 177(6):3657-68 (2006)
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-5143.idf.txt
Sample and data relationshipE-GEOD-5143.sdrf.txt
Processed data (1)E-GEOD-5143.processed.1.zip
Array designA-AFFY-44.adf.txt
Links