E-GEOD-51394 - Wildtype, miR-1-1 KO, miR-1 Double het P2 mixed strain heart analysis (MoGene 2.0 ST Arrays).
Released on 19 October 2013, last updated on 28 October 2013
Neonatal hearts (P2) from wildtype, miR-1-1 null and miR-1-2 +/-: miR-1-1 +/- double heterozygote animals were isolated and total RNA was extracted with TRIzol (Invitrogen), following the manufacturer’s suggested protocol. Genomic DNA was removed using a gDNA eliminator column (Qiagen). Samples were hybridized to Affymetrix mouse genechip ST 2.0 arrays. Mouse strain for all samples is: Mixed 129 x C57BL/6 Three groups: miR-1-1, miR-1 double het, wildtype, all compared against each other. Samples are all from neonatal hearts.
transcription profiling by array
Alex Williams <firstname.lastname@example.org>, Amy Heidersbach