E-GEOD-51393 - A microRNA-directed program of cytotoxic CD8+ T cell differentiation

Status
Released on 2 December 2013, last updated on 3 June 2014
Organism
Mus musculus
Samples (12)
Protocols (2)
Description
Acquisition of effector properties is a key step in the generation of cytotoxic T lymphocytes (CTLs). Here we show that inflammatory signals regulate Dicer expression in CTL, and that deletion or depletion of Dicer in mouse or human activated CD8+ T cells causes upregulation of perforin, granzyme and effector cytokines. Genome-wide analysis of miRNA changes induced by exposure of differentiating CTLs to IL-2 and inflammatory signals identifies miR-139 and miR-150 as components of a miRNA network that controls perforin, eomesodermin (Eomes) and IL-2Ra expression in differentiating CTLs and whose activity is modulated by IL-2, inflammation and antigenic stimulation. Overall our data show that strong IL-2R and inflammatory signals act through Dicer and miRNAs to control the cytolytic program and other aspects of effector CTL differentiation. Comparison of control and Dicer knock-out CTLs differentiated in vitro; Comparison of wild type CTLs differentiated in vitro with or without inflammatory stimuli; Comparison of effector and memory precursor CTLs isolated from mice infected with LCMV-Armstrong
Experiment type
RNA-seq of non coding RNA 
Contacts
Tarmo Äijö <geo@ncbi.nlm.nih.gov>, Anjana Rao, Sara Trifari
Citation
MicroRNA-directed program of cytotoxic CD8+ T-cell differentiation. Trifari S, Pipkin ME, Bandukwala HS, Aij� T, Bassein J, Chen R, Martinez GJ, Rao A. , Europe PMC 24163352
MINSEQE
Exp. designProtocolsVariablesProcessedSeq. reads
Files
Investigation descriptionE-GEOD-51393.idf.txt
Sample and data relationshipE-GEOD-51393.sdrf.txt
Processed data (1)E-GEOD-51393.processed.1.zip
Links