E-GEOD-51309 - Age-related changes in microRNA levels in serum (mRNA)
Released on 9 October 2013, last updated on 3 June 2014
Age-related changes in microRNA levels in serum 20 nM biotinylated miRIDIAN miR-1248 mimic (Thermo Scientific) or a negative control, biotinylated miRIDIAN C. elegans miR-67 (here named Con-miR) using Lipofectamine 2000. Forty eight hours post transfection, total RNA was isolated using the mirVana kit (Invitrogen) according to manufacturer’s procedure. Three replicate experiments were performed and a technical repeat was included for each sample. Samples were analyzed using Illumina's Sentrix Human HT-12 ver4 Expression BeadChips (Illumina, San Diego, CA).
transcription profiling by array
Kevin G Becker <firstname.lastname@example.org>, Alan B Zonderman, Julie A Mattison, Megan Fitzpatrick, Michele K Evans, Ngozi Ejiogu, Nicole N Hooten, Supriyo De, William H Wood, Yongqing Zhang
Age-related changes in microRNA levels in serum. Noren Hooten N, Fitzpatrick M, Wood WH 3rd, De S, Ejiogu N, Zhang Y, Mattison JA, Becker KG, Zonderman AB, Evans MK.