E-GEOD-51309 - Age-related changes in microRNA levels in serum (mRNA)

Status
Released on 9 October 2013, last updated on 3 June 2014
Organism
Homo sapiens
Samples (12)
Array (1)
Protocols (7)
Description
Age-related changes in microRNA levels in serum 20 nM biotinylated miRIDIAN miR-1248 mimic (Thermo Scientific) or a negative control, biotinylated miRIDIAN C. elegans miR-67 (here named Con-miR) using Lipofectamine 2000. Forty eight hours post transfection, total RNA was isolated using the mirVana kit (Invitrogen) according to manufacturer’s procedure. Three replicate experiments were performed and a technical repeat was included for each sample. Samples were analyzed using Illumina's Sentrix Human HT-12 ver4 Expression BeadChips (Illumina, San Diego, CA).
Experiment type
transcription profiling by array 
Contacts
Kevin G Becker <beckerk@grc.nia.nih.gov>, Alan B Zonderman, Julie A Mattison, Megan Fitzpatrick, Michele K Evans, Ngozi Ejiogu, Nicole N Hooten, Supriyo De, William H Wood, Yongqing Zhang
Citation
Age-related changes in microRNA levels in serum. Noren Hooten N, Fitzpatrick M, Wood WH 3rd, De S, Ejiogu N, Zhang Y, Mattison JA, Becker KG, Zonderman AB, Evans MK.
MIAME
PlatformsProtocolsFactorsProcessedRaw
Files
Investigation descriptionE-GEOD-51309.idf.txt
Sample and data relationshipE-GEOD-51309.sdrf.txt
Processed data (1)E-GEOD-51309.processed.1.zip
Array designA-GEOD-10558.adf.txt
Links