E-GEOD-5124 - Transcription profiling of mouse rhombomere 4 at E10.5 from knockins of Hoxb1 gene expressing Hox-A1 protein

Status
Submitted on 22 June 2006, released on 13 June 2008, last updated on 27 March 2012
Organism
Mus musculus
Samples (6)
Array (1)
Protocols (2)
Description
This study analyzed mRNA profiles in rhombomere 4 of E10.5 mouse knock-in embryos expressing either normal endogenous Hox-B1 protein or the paralogous Hox-A1 protein from the Hoxb1 locus. The Hox-A1 protein was found to be detectably less efficacious than Hox-B1 in promoting neurogenesis in the basal plate of rhombomere 4 and its transcriptional profile shared several similarities with the Hoxb1 mutant. Experiment Overall Design: GFP-positive cells were FACS-sorted from dissected hindbrains of entire litters of E10.5 mouse embryos expressing either normal endogenous Hox-B1 protein or the paralogous Hox-A1 protein from the Hoxb1 locus, either one tagged with IRES-tauGFP. Three independent biological replicates of each genotype were analyzed. Total RNA was isolated, amplified and hybridized to Affymetrix Mouse Genome 430 2.0 Arrays.
Experiment types
transcription profiling by array, unknown experiment type
Contact
Citation
Reversal of Hox1 gene subfunctionalization in the mouse. Petr Tvrdik, Mario R Capecchi. Dev Cell 11(2):239-50 (2006)
MIAME
PlatformsProtocolsFactorsProcessedRaw
Files
Investigation descriptionE-GEOD-5124.idf.txt
Sample and data relationshipE-GEOD-5124.sdrf.txt
Raw data (1)E-GEOD-5124.raw.1.zip
Processed data (1)E-GEOD-5124.processed.1.zip
Array designA-AFFY-45.adf.txt
R ExpressionSetE-GEOD-5124.eSet.r
Links