E-GEOD-51145 - Highly efficient methane biocatalysis revealed in a methanotrophic bacterium

Released on 25 September 2013, last updated on 9 October 2013
Methylomicrobium alcaliphilum 20Z
Samples (8)
Protocols (3)
RNA-Seq profiling of Methylomicrobium alcaliphilum strain 20Z grown in batch on methane. The RNA-Seq work is one part of a systems approach to characterizing metabolism of 20Z during growth on methane. We demonstrate that methane assimilation is coupled with a highly efficient pyrophosphate-mediated glycolytic pathway, which under O2 limitation participates in a novel form of fermentation-based methanotrophy. This surprising discovery suggests a novel mode of methane utilization in oxygen-limited environments, and opens new opportunities for a modular approach towards producing a variety of excreted chemical products using methane as a feedstock. Four replicates of batch growth
Experiment type
RNA-seq of coding RNA 
David A. C. Beck <dacb@uw.edu>, David A Beck, Marina G Kalyuzhnaya
Exp. designProtocolsVariablesProcessedSeq. reads
Investigation descriptionE-GEOD-51145.idf.txt
Sample and data relationshipE-GEOD-51145.sdrf.txt