ArrayExpress

Released on 25 September 2013, last updated on 30 September 2013

Methylomicrobium alcaliphilum 20Z

RNA-Seq profiling of Methylomicrobium alcaliphilum strain 20Z grown in batch on methane. The RNA-Seq work is one part of a systems approach to characterizing metabolism of 20Z during growth on methane. We demonstrate that methane assimilation is coupled with a highly efficient pyrophosphate-mediated glycolytic pathway, which under O2 limitation participates in a novel form of fermentation-based methanotrophy. This surprising discovery suggests a novel mode of methane utilization in oxygen-limited environments, and opens new opportunities for a modular approach towards producing a variety of excreted chemical products using methane as a feedstock. Four replicates of batch growth

RNA-seq of coding RNA 

David A. C. Beck <dacb@uw.edu>, David A Beck, Marina G Kalyuzhnaya

ENA - SRP030410, GEO - GSE51145