E-GEOD-51102 - Autoimmune antigen and serum factor antigen reactivity in a cohort of patients with mycobacterium infection shown previously to be positive and negative for IgG autoantibodies to IFN-gamma.

Status
Released on 24 September 2013, last updated on 30 September 2013
Organism
Homo sapiens
Samples (10)
Array (1)
Protocols (7)
Description
The aim of the study was to test wether our microarray platform is capable of detecting previously described IgG autoantibodies to IFN-gamma. We diluted patient sera 1:250 and incubated dilutions on a nitrocellulose-platform array printed with whole protein antigens. In this study, serum from 10 individuals (subsetted into two groups: individuals positive or negative for previously described IFN-gamma-targeted autoantibodies) was profiled for IgG autoantibody reactivity to whole protein autoimmune and serum factor antigens. We found that IFN-gamma-targeted IgG autoantibodies were recognized in the IFNg-pos samples and also identified other array targets significantly associated with anti-IFNg positive individials using the significance analysis of microarrays (SAM) algorithm.
Experiment type
other 
Contacts
Jordan Price <geo@ncbi.nlm.nih.gov>, Jordan V Price, Paul J Utz
MIAME
PlatformsProtocolsFactorsProcessedRaw
Files
Investigation descriptionE-GEOD-51102.idf.txt
Sample and data relationshipE-GEOD-51102.sdrf.txt
Raw data (1)E-GEOD-51102.raw.1.zip
Processed data (1)E-GEOD-51102.processed.1.zip
Array designA-GEOD-17754.adf.txt
Links