E-GEOD-51101 - Autoimmune antigen and serum factor antigen reactivity in a cohort of patients with autoimmune polyendocrinopathy syndrome type 1(APS-1) and healthy controls.

Status
Released on 24 September 2013, last updated on 30 September 2013
Organism
Homo sapiens
Samples (14)
Array (1)
Protocols (7)
Description
The aim of the study was to test the hypothesis that APS-1 patient sera contains IgG reactivity to serum factor antigens concomitant with prototypical autoimmne antigens. We diluted patient sera 1:250 and incubated dilutions on a nitrocellulose-platform array printed with whole protein antigens. In this study, serum from 14 ndividuals (subsetted into APS-1 patient and healthy control categories) was profiled for IgG autoantibody reactivity to whole protein autoimmune and serum factor antigens. APS-1 patient sera was found using the significance analysis of microarrays (SAM) algorithm to be significantly more reactive with prototypical cytokine antigens previously identified as targets of autoantibodies.
Experiment type
other 
Contacts
Jordan Price <geo@ncbi.nlm.nih.gov>, Jordan V Price, Paul J Utz
MIAME
PlatformsProtocolsFactorsProcessedRaw
Files
Investigation descriptionE-GEOD-51101.idf.txt
Sample and data relationshipE-GEOD-51101.sdrf.txt
Raw data (1)E-GEOD-51101.raw.1.zip
Processed data (1)E-GEOD-51101.processed.1.zip
Array designA-GEOD-17753.adf.txt
Links