E-GEOD-51100 - Autoimmune antigen and serum factor antigen reactivity in a cohort of patients with systemic lupus erythematosus (SLE) and healthy controls from the ABCoN cohort.

Status
Released on 24 September 2013, last updated on 30 September 2013
Organism
Homo sapiens
Samples (45)
Array (1)
Protocols (7)
Description
The aim of the study was to test the hypothesis that SLE patient sera contains IgG reactivity to serum factor antigens concomitant with prototypical autoimmne antigens. We diluted patient sera 1:250 and incubated dilutions on a nitrocellulose-platform array printed with whole protein antigens. In this study, serum from 45 individuals (subsetted into high and low interferon signature as well as healthy control categories) was profiled for IgG autoantibody reactivity to whole protein autoimmune and serum factor antigens. SLE and specifically IFN high patient sera was found using the significance analysis of microarrays (SAM) algorithm to be significantly more reactive with prototypical autoimmune antigens and select serum factor array antigens.
Experiment type
other 
Contacts
Jordan Price <geo@ncbi.nlm.nih.gov>, Jordan V Price, Paul J Utz
MIAME
PlatformsProtocolsFactorsProcessedRaw
Files
Investigation descriptionE-GEOD-51100.idf.txt
Sample and data relationshipE-GEOD-51100.sdrf.txt
Raw data (1)E-GEOD-51100.raw.1.zip
Processed data (1)E-GEOD-51100.processed.1.zip
Array designA-GEOD-17752.adf.txt
Links