E-GEOD-51099 - Expression data from Ctla4-/- and DKO CD4+ Tconv cells

Status
Released on 25 September 2013, last updated on 30 September 2013
Organism
Mus musculus
Samples (4)
Array (1)
Protocols (6)
Description
Ctla4-/- mice suffer from a severe autoimmunity characterized by indiscriminate self-reactive T cell activation. Itk-/-Ctla4-/- (DKO) mice are protected from lethal autoimmunity despite a fulminant autoimmune process in the LNs as self-reative T cells fail to migrate to destroy tissues. We used microarray to identify underlying differences in gene expression that could account for lack of migration of DKO T cells into tissues. CD4+CD25neg T cells from 3 weeks old male Ctla4-/- or DKO mice were sorted in duplicates, RNA was extracted using TRIzol reagent and microarray analysis performed with the Affymetrix MoGene 1.0 ST array
Experiment type
transcription profiling by array 
Contacts
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-51099.idf.txt
Sample and data relationshipE-GEOD-51099.sdrf.txt
Raw data (1)E-GEOD-51099.raw.1.zip
Processed data (1)E-GEOD-51099.processed.1.zip
Array designA-AFFY-130.adf.txt
Links