E-GEOD-51075 - Transcriptional responses of murine macrophages to the adenylate cyclase toxin of Bordetella pertussis

Status
Released on 22 September 2013, last updated on 3 June 2014
Organism
Mus musculus
Samples (12)
Array (1)
Protocols (7)
Description
Three different recombinant forms of CyaA were used to investigate transcriptional responses of murine bone marrow-derived macrophages (BMDMs) using Affymetrix Mouse Genome Genechips. These forms were enzymically active, invasive CyaA, nonenzymically active, invasive CyaA (CyaA*) and non-enzymically active, non-invasive CyaA (proCyaA*). BMMs, treated with 20 ng/ml of CyaA for 24 h, showed over 1000 significant changes in gene transcription compared with control cells. CyaA caused an increase in transcription of many inflammatory genes and genes associated with various signalling cascades such as those involved in cyclic AMP-dependent protein kinase A signalling. Most strikingly, CyaA caused down-regulation of numerous genes involved in cell proliferation. CyaA* at 20 ng/ml significantly up-regulated the transcription of only twelve genes after 24 h whereas proCyaA* at this concentration significantly increased the transcription of only two genes. The effect of administration of three different recombinant forms of pertussis toxin to mouse bone marrow derived macrophages are compared with a vehicle only contol 24 hours after treatment in technical triplicate measurements
Experiment type
transcription profiling by array 
Contacts
Gordon Y Cheung, Nigel Binns
Citation
MIAME
PlatformsProtocolsFactorsProcessedRaw
Files
Investigation descriptionE-GEOD-51075.idf.txt
Sample and data relationshipE-GEOD-51075.sdrf.txt
Raw data (1)E-GEOD-51075.raw.1.zip
Processed data (1)E-GEOD-51075.processed.1.zip
Array designA-AFFY-45.adf.txt
Links