E-GEOD-51073 - Expression data from non-pigmented and pigmented mouse melanocytes

Status
Released on 21 September 2013, last updated on 3 June 2014
Organism
Mus musculus
Samples (4)
Array (1)
Protocols (7)
Description
Immortalized, amelanotic melanocytes isolted from skin of Balb/c express enzymatically-inactive tyrosinase due to a homozygous point mutation (TGT->TCT) in tyrosinase gene, resulting in a lack of melanin . To serve as a control cell line, pigmentation was restored in these cells by correcting the point mutation using an RNA-DNA oligonucleotide (kingly gift from Dr. Alexeev Y. Vitali). We used microarray to detail the effect of tyrosinase mutation on gene expression in normal versus mutant melanocytes. Pigmented and non-pigmented melanocytes were grown for RNA extraction and hybridization on Affymetrix microarrays. We used both normal and mutant melanocyte in order to obtain expression profiles. We then examined variances in gene expression between the two cell lines.
Experiment type
transcription profiling by array 
Contacts
Irit Adini, Robert J D'Amato
MIAME
PlatformsProtocolsFactorsProcessedRaw
Files
Investigation descriptionE-GEOD-51073.idf.txt
Sample and data relationshipE-GEOD-51073.sdrf.txt
Raw data (1)E-GEOD-51073.raw.1.zip
Processed data (1)E-GEOD-51073.processed.1.zip
Array designA-AFFY-45.adf.txt
Links