E-GEOD-50985 - Transcriptomic analysis of a nickel-resistant Saccharomyces cerevisiae mutant obtained by evolutionary engineering

Status
Released on 19 September 2013, last updated on 30 September 2013
Organism
Saccharomyces cerevisiae
Samples (6)
Array (1)
Protocols (7)
Description
Nickel-resistant Saccharomyces cerevisiae mutant was obtained by evolutionary engineering. The reference strain which was used to select this nickel-resistant mutant could not grow even at 0.5 mM NiCl2 whereas this mutant was shown to be resistant upto 5.3 mM NiCl2 concentration. Whole-genome microarray analysis might be promising to identify the nickel resistance mechanisms in the yeast cells. The reference Saccharomyces cerevisiae strain and the nickel-resistant mutant were grown in minimal medium to an Optical Density (OD600) of 1.00 which correspond to the logarithmic growth phase of the yeast cells. Cultures were harvested and whole RNA isolation was carried out. The experiment was repeated three times.
Experiment type
transcription profiling by array 
Contacts
CEREN ALKIM <alkim@insa-toulouse.fr>, C Alkım, G Küçükgöze, H I Kısakesen, U Yılmaz, Z P Cakar
MIAME
PlatformsProtocolsFactorsProcessedRaw
Files
Investigation descriptionE-GEOD-50985.idf.txt
Sample and data relationshipE-GEOD-50985.sdrf.txt
Processed data (1)E-GEOD-50985.processed.1.zip
Array designA-GEOD-16244.adf.txt
Links