E-GEOD-50981 - Transcriptional changes in Zea mays seedlings treated with insect elicitor (volicitin).

Status
Released on 19 September 2013, last updated on 2 May 2014
Organism
Zea mays
Samples (8)
Array (1)
Protocols (7)
Description
Insect elicitors, in particular fatty acid amides like volicitin, have been known to induce defense-related gene expression. Here we investigated transcriptional changes in response to volicitin 60min after treatment locally and in distal parts of the treated leaf. 3-Week old Zea mays seedling (inbred line B73) were treated with pure volicitin (1nmol per plant). Controls were untreated. Plants were for 60 min. The second leaf was then taken and a segment of 1 cm cut around the damage site and shock-frozen in liquid N2. 3 segments from 3 individual leaves were pooled for one biological replicate and then stored at -85°prior to RNA extraction. For distal gene expression analysis a 1cm segment was cut about 3 cm leaf upwards from the volicitin application site and also frozen in liquid N2. 3 segments from 3 individual leaves were pooled for one biological replicate and then stored at -85°prior to RNA extraction. 2 biological replicates were performed for each treatment with one dye swap (second sample).
Experiment type
transcription profiling by array 
Contacts
MIAME
PlatformsProtocolsFactorsProcessedRaw
Files
Investigation descriptionE-GEOD-50981.idf.txt
Sample and data relationshipE-GEOD-50981.sdrf.txt
Raw data (1)E-GEOD-50981.raw.1.zip
Processed data (1)E-GEOD-50981.processed.1.zip
Array designA-GEOD-6438.adf.txt
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