E-GEOD-50979 - Direct comparison of effects of slow-freezing and vitrification procedures on rabbit late blastocyst transcriptome

Status
Released on 19 September 2013, last updated on 3 June 2014
Organism
Oryctolagus cuniculus
Samples (6)
Array (1)
Protocols (6)
Description
Nowadays there is a need to improve cryopreservation protocols in both human assisted reproduction and domestic animal field. We wanted to compare the transcriptomic changes induced by two commonly used cryopreservation procedures (slow freezing and vitrification) on rabbit late blastocyst before the onset of implantation. We recovered rabbit morulae at day 3 of development, freezing or vitrified and transferred them into recipient maternal tracts till day 6 of development. In this way, we wanted to analyse if different cryopreservation techniques produce different effects on gene expression that embryos are unable to get over after three days of in vivo development. After artificially insemination morulae were recovered at day 3 of development, frozen or vitrified, and then transferred to recipient does. To assess gene expression differences between frozen and vitrified the recipients were slaughtered three days after the transfer, at day 6.
Experiment type
transcription profiling by array 
Contacts
M. Desemparats Saenz-de-Juano <masaede@upvnet.upv.es>, F Marco-Jimenez, Jose S Vicente, M D Saenz-de-Juano
MIAME
PlatformsProtocolsFactorsProcessedRaw
Files
Investigation descriptionE-GEOD-50979.idf.txt
Sample and data relationshipE-GEOD-50979.sdrf.txt
Raw data (1)E-GEOD-50979.raw.1.zip
Processed data (1)E-GEOD-50979.processed.1.zip
Array designA-GEOD-13288.adf.txt
Links