normalization data transformation protocol
Illumina Casava1.8.2 software used for basecalling. Sequenced reads were mapped to GRCh3 using tophat v2.0.8 FPKM values were obtained from the tophat output Genome_build: GRCh37 Supplementary_files_format_and_content: Tab-delimited text files include FPKM values for genes and transcript isoforms, and read counts per gene.
nucleic acid library construction protocol
Total RNA was extracted from whole blood, and depleted of rRNA (Ribo-zero; Epicentre) and globin RNA (Globinclear; Life Technologies) before amplification (Ovation; Nugen). RNA libraries were prepared for sequencing using standard Illumina protocols