2 protocols
AccessionNameType
P-GSE50957-2
normalization data transformation protocol
Illumina Casava1.8.2 software used for basecalling. Sequenced reads were mapped to GRCh3 using tophat v2.0.8 FPKM values were obtained from the tophat output Genome_build: GRCh37 Supplementary_files_format_and_content: Tab-delimited text files include FPKM values for genes and transcript isoforms, and read counts per gene.
P-GSE50957-1
nucleic acid library construction protocol
Total RNA was extracted from whole blood, and depleted of rRNA (Ribo-zero; Epicentre) and globin RNA (Globinclear; Life Technologies) before amplification (Ovation; Nugen). RNA libraries were prepared for sequencing using standard Illumina protocols