E-GEOD-5054 - Transcription profiling of human thyroid cells exposed to INF-gamma and IL1-beta

Status
Submitted on 12 June 2006, released on 6 November 2008, last updated on 27 March 2012
Organism
Homo sapiens
Samples (20)
Arrays (2)
Protocols (2)
Description
Normal primary thyroid cells were incubated with , 100 IU/ml IFN-gamma, 50 IU/ml IL1-beta, or a combination of both IFN-gamma and IL1-beta for 24 or 72 hours. The experiment was repeated 5 times using thyroid cells from 5 different patients. RNA expression was analyzed using Affymetrix HG_U133A arrays for 3 of the thyroids, and HG_U133A_2.0 (small version of HG_U133A) arrays for 2 of the thyroids. Experiment Overall Design: Normal primary thyroid cells were incubated with , 100 IU/ml IFN-gamma, 50 IU/ml IL1-beta, or a combination of both IFN-gamma and IL1-beta for 24 or 72 hours. The experiment was repeated 5 times using thyroid cells from 5 different patients. One array was hybridized per sample (20 samples). Experiment Overall Design: The probe-set intensities with accompanying statistical analysis (2-way ANOVA) and the associated README file are included as Supplementary files.
Experiment types
transcription profiling by array, compound treatment, individual comparison
Contact
Citation
Microarray analysis of cytokine activation of apoptosis pathways in the thyroid. Su He Wang, Mary Van Antwerp, Rork Kuick, Paul G Gauger, Gerard M Doherty, Yang Yi Fan, James R Baker.
MIAME
PlatformsProtocolsFactorsProcessedRaw
Files
Investigation descriptionE-GEOD-5054.idf.txt
Sample and data relationshipE-GEOD-5054.sdrf.txt
Raw data (1)E-GEOD-5054.raw.1.zip
Processed data (1)E-GEOD-5054.processed.1.zip
Array designsA-AFFY-33.adf.txt, A-AFFY-37.adf.txt
R ExpressionSetE-GEOD-5054.eSet.r
Links