E-GEOD-50162 - Genome wide gene-expression analysis of the spider mite Tetranychus urticae after long term host transfer from acyanogenic Phaseolus vulgaris cv. 'Prelude' bean plants to cyanogenic Phaseolus lunatus cv. '8078' bean plants

Status
Released on 30 April 2014, last updated on 3 June 2014
Organism
Tetranychus urticae
Samples (8)
Array (1)
Protocols (6)
Description
250 adult T. urticae females from the London strain (grown on acyanogenic P. vulgaris cv. Prelude bean plants) were transferred to cyanogenic P. lunatus cv. 8078 bean plants. Thirty-five generations after the host transfer, total RNA was extracted from mites growing on both bean species (London and London-CYANO strain) and used in in a genome-wide gene expression microarray (Sureprint G3 microarray, Agilent) experiment to assess significantly differentially expressed genes (FC ≥ 2 and FDR-corrected p-value < 0.05) between mites grown on P. vulgaris (cv. Prelude) bean plants (London strain) and mites grown for 35 generations on P. lunatus (cv. 8078) bean plants (London-CYANO strain). 4 replicates for one comparison: mites of the London strain grown on P. lunatus for 35 generations (London-CYANO) compared to mites of the London strain grown on P. vulgaris bean plants (London)
Experiment type
transcription profiling by array 
Contacts
Nicky Wybouw, Thomas Van Leeuwen, Wannes Dermauw
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-50162.idf.txt
Sample and data relationshipE-GEOD-50162.sdrf.txt
Raw data (1)E-GEOD-50162.raw.1.zip
Processed data (1)E-GEOD-50162.processed.1.zip
Array designA-GEOD-16890.adf.txt
Links