normalization data transformation protocol
The data were analyzed with Partek Genomics Suite software (v.6.5) using default analysis settings and normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100. ID_REF = VALUE = log2 normalized
array scanning protocol
GeneChips were scanned using the Affymetrix ® GeneChip® Scanner 3000.
Following fragmentation and preparation, 10μg of cRNA were hybridized for 16 hr at 45℃ on Affymetrix Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1μg total RNA (GeneChip® Expression Analysis Technical Manual, 2001, Affymetrix).
nucleic acid extraction protocol
Trizol extraction of total RNA from enriched T-cells was performed according to the manufacturer's instructions.