7 protocols
AccessionNameType
P-GSE49855-1
normalization data transformation protocol
Array Pro software was used to calculate differences in signal intensities and to normalize by Z-score transformation ID_REF = VALUE = Averaged z data
P-GSE49855-7
array scanning protocol
the submiitter has not supplied the information
P-GSE49855-6
hybridization protocol
RNA was denatured with NaOH and spotted onto S&S Nytran SuperCharge nylon membranes. Twenty-four 384-well plates were printed using a BioRobotics MicroGrid Arrayer.
P-GSE49855-5
labelling protocol
RNA was reverse transcribed in the presence of [α-33P]dCTP.
P-GSE49855-4
nucleic acid extraction protocol
RNA was extracted with phenol/chloroform from C2C12 cell extracts that were subject to immunoprecipitation with an antibody that recognized either HuR or IgG.
P-GSE49855-2
sample treatment protocol
No treatment
P-GSE49855-3
growth protocol
Proliferating C2C12 muscle cells were grown in DMEM supplemented with 20% FBS and 1% Pen/Strep. At 80% confluency, cells were collected.