E-GEOD-49627 - Large-scale hypomethylated blocks associated with Epstein-Barr virus-induced B-cell immortalization [Bisulfite-seq]

Status
Released on 17 September 2013, last updated on 3 October 2013
Organism
Homo sapiens
Samples (15)
Protocols (3)
Description
To determine what DNA methylation and gene expression changes occur following EBV transformation. B-cells were isolated from 3 donors. Resting, CD40 activated and EBV transfromed cells from each donor was analyzed. Each sample was assayed using Affymetrix expression arrays and whole genome bisulfite sequenicng. Additional time points during transformation and activation were sequenced as well, but not assayed for expression. B-cells were isolated from 3 donors. Resting, CD40 activated and EBV transfromed cells from each donor was analyzed. Additional B-cells were isolated from 2 additional donors and assay at days 16 and week 3 following CD40 activation and EBV infection. Only one donor contributed to the EBV timepoints.
Experiment type
methylation profiling by high throughput sequencing 
Contacts
Kasper Daniel Hansen <kasperdanielhansen@gmail.com>, Kasper D Hansen, Sarven Sarbunciyan
MINSEQE
Exp. designProtocolsFactorsProcessedSeq. reads
Files
Investigation descriptionE-GEOD-49627.idf.txt
Sample and data relationshipE-GEOD-49627.sdrf.txt
Processed data (15)Click to browse processed data
Links