E-GEOD-49619 - Expression data from CD4+ Naïve T cells under Treg-inducing condition in the presence or absence of butyrate

Status
Released on 20 November 2013, last updated on 25 November 2013
Organism
Mus musculus
Samples (4)
Array (1)
Protocols (7)
Description
Commensal bacteria shapes gut immune system. Colonization bacteria increase the frequency of regulatory T cells, however, the molecular mechanisms has not yet been unknown. To reveal the mechanism, we isolated Naïve CD4+ T cells from spleen of C57BL/6 mice and cultured the cells under Treg-inducing condition culture in the presence or absence of butyrate, a metabolite produced by commensal bacteria. Naïve T cells were isolated from spleen and were cultured in the presence of IL-2, TGF-beta and in the presence or absene of Butyrate. RNA was extracted at Day 2
Experiment type
transcription profiling by array 
Contacts
Takaho A. Endo <takaho.endo@riken.jp>, Koji Hase, Takaho A Endo, Yukihiro Furusawa
Citation
Commensal microbe-derived butyrate induces the differentiation of colonic regulatory T cells. Furusawa Y, Obata Y, Fukuda S, Endo TA, Nakato G, Takahashi D, Nakanishi Y, Uetake C, Kato K, Kato T, Takahashi M, Fukuda NN, Murakami S, Miyauchi E, Hino S, Atarashi K, Onawa S, Fujimura Y, Lockett T, Clarke JM, Topping DL, Tomita M, Hori S, Ohara O, Morita T, Koseki H, Kikuchi J, Honda K, Hase K, Ohno H. , Europe PMC 24226770
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-49619.idf.txt
Sample and data relationshipE-GEOD-49619.sdrf.txt
Raw data (1)E-GEOD-49619.raw.1.zip
Processed data (1)E-GEOD-49619.processed.1.zip
Array designA-AFFY-130.adf.txt
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