E-GEOD-49510 - Global gene expression pattern difference between the high density and the regular density cultured rat bone marrow cells

Status
Released on 3 August 2013, last updated on 3 June 2014
Organism
Rattus norvegicus
Samples (4)
Array (1)
Protocols (6)
Description
In vitro expansion of endothelial progenitor cells (EPCs) remains a challenge in stem cell research and its application. We hypothesize that high density culture is able to expansion EPCs from bone marrow by mimicking cell-cell interactions of the bone marrow niche. To test the hypothesis, rat bone marrow cells were either cultured in high density by seeding or cultured in regular density. Flow cytometric analyses of the cells cultured for 15 days showed that high density cells exhibited smaller cell size and higher levels of marker expression related to EPCs when compared to regular density cultured cells. Global gene expression pattern difference between the high density and the regular density cultured cells was analyzed by a microarray assay. Gene expression in rat bone marrow cells was measured at 15 days after high density culture or regular density cluture. 2 independent experiments were performed using different high density(HD) cultured cells for each experiment and bone marrow cells in regular density(RD) were used as a control.
Experiment type
transcription profiling by array 
Contacts
MIAME
PlatformsProtocolsFactorsProcessedRaw
Files
Investigation descriptionE-GEOD-49510.idf.txt
Sample and data relationshipE-GEOD-49510.sdrf.txt
Processed data (1)E-GEOD-49510.processed.1.zip
Array designA-GEOD-14746.adf.txt
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