E-GEOD-49477 - Semiconductor based DNA sequencing of histone modification states

Status
Released on 25 October 2013, last updated on 25 November 2013
Organism
Homo sapiens, Mus musculus
Samples (17)
Protocols (4)
Description
The recent development of a semiconductor-based, non-optical DNA sequencing technology promises scalable, low-cost and rapid sequence data production. The technology has previously been applied mainly to genomic sequencing and targeted re-sequencing. Here, we demonstrate the utility of Ion Torrent semiconductor-based sequencing for sensitive, efficient and rapid chromatin immunoprecipitation followed by sequencing (ChIP-seq) through the application of sample preparation methods that are optimized for ChIP-seq on the Ion Torrent platform. We leverage this method for epigenetic profiling of tumor tissues. Examination of histone modifications in mouse dendentic cells stimulated with LPS, matched melanoma derived cell line, melanoma tumor tissue
Experiment type
ChIP-seq 
Contacts
Christine S Cheng, Ido Amit
MINSEQE
Exp. designProtocolsFactorsProcessedSeq. reads
Files
Investigation descriptionE-GEOD-49477.idf.txt
Sample and data relationshipE-GEOD-49477.sdrf.txt
Processed data (1)E-GEOD-49477.processed.1.zip
Links