E-GEOD-49216 - Gene regulation by Pax6 in the lens placode - E10.25

Status
Released on 23 September 2013, last updated on 3 June 2014
Organism
Mus musculus
Samples (6)
Array (1)
Protocols (7)
Description
Wild type or Pax6 fx/fx; Le-Cre-positive surface ectoderm from E10.25 mouse embryos was laser micodissected from three embryos of each genotype. Total RNA was purified and reverse transcribed and amplified using a NuGEN kit. cDNA was biotinylated and hybridized to Illumina Mouse6 bead arrays. Three wild type and three knockout embryos were used. Each array used the cDNA obtained from the two prospective lens tissues from one embryo.
Experiment type
transcription profiling by array 
Contacts
David Cy Beebe <beebe@wustl.edu>, David C Beebe, Jie Huang
Citations
Histone posttranslational modifications and cell fate determination: lens induction requires the lysine acetyltransferases CBP and p300. Wolf L, Harrison W, Huang J, Xie Q, Xiao N, Sun J, Kong L, Lachke SA, Kuracha MR, Govindarajan V, Brindle PK, Ashery-Padan R, Beebe DC, Overbeek PA, Cvekl A. , Europe PMC 24038357
The mechanism of lens placode formation: a case of matrix-mediated morphogenesis. Huang J, Rajagopal R, Liu Y, Dattilo LK, Shaham O, Ashery-Padan R, Beebe DC. , Europe PMC 21540023
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-49216.idf.txt
Sample and data relationshipE-GEOD-49216.sdrf.txt
Processed data (1)E-GEOD-49216.processed.1.zip
Array designA-MEXP-933.adf.txt
Links