E-GEOD-49082 - Genomic alteration in MRC5-derived induced pluripotent stem (MRC5-iPS) cell line, Tic

Status
Released on 23 July 2013, last updated on 3 June 2014
Organism
Homo sapiens
Samples (4)
Array (1)
Protocols (6)
Description
Disease-specific induced pluripotent stem (iPS) cells have been used for a model to analyze pathogenesis of the disease. We generated iPS cells derived from a fibroblastic cell line of ataxia telangiectasia (AT-iPS cells). In analysis of AT-iPS cells, the human wild-type iPS cell line (MRC5-iPS) was generated and cultured in the same conditions as the diseased iPS cell lines. It is an ideal control cell line for the disease and patient-specific iPS cell lines. Because MRC5-iPS cells exhibited considerable chromosomal abnormalities in vitro, we performed a structural alteration analysis by using a SNP genotyping array for MRC5-iPS cell line, Tic, at passage 15, passage 30, and passage 37. The parental MRC-5 fibroblast cells and MRC-iPS 25 (Tic) were subjected to Illumina HumanCytoSNP-12 v2.1 BeadChip analysis.
Experiment types
genotyping by array, comparative genomic hybridization by array 
Contacts
Akihiro Umezawa, Kazuhiro Nakabayashi, Kenichiro Hata, Kohji Okamura, Toyoda Masashi
MIAME
PlatformsProtocolsFactorsProcessedRaw
Files
Investigation descriptionE-GEOD-49082.idf.txt
Sample and data relationshipE-GEOD-49082.sdrf.txt
Processed data (1)E-GEOD-49082.processed.1.zip
Array designA-GEOD-13829.adf.txt
Links