E-GEOD-49077 - Transcriptome analysis of the D3 mouse embryonic stem cell line in comparison to the MIN6 pancreatic beta-cell insulinoma cell line
Released on 23 July 2013, last updated on 29 July 2013
Analysis of the transcriptional differences between mouse ES cells from the D3 cell line and transformed beta-cells (MIN6 cell line) as determined by a direct comparative analysis of their transcriptome. The results show that 40% of transcripts were differentially expressed between D3 and MIN6 cells. There is thus a marked difference in the pattern of transcription between the ES cell and beta-cell genomes. Total RNA was extracted from three independent replicates of passage 21 D3 cells and passage 23 MIN6 cells. A whole mouse genome comparison between D3 and MIN6 cells was performed using the Mouse WG-6 v2.0 Expression Beadchip from Illumina.
transcription profiling by array
Chris O'Neill <firstname.lastname@example.org>, Christopher O'Neill, Jennifer Y Wong, Michelle M Jack