E-GEOD-49077 - Transcriptome analysis of the D3 mouse embryonic stem cell line in comparison to the MIN6 pancreatic beta-cell insulinoma cell line

Status
Released on 23 July 2013, last updated on 3 June 2014
Organism
Mus musculus
Samples (6)
Array (1)
Protocols (5)
Description
Analysis of the transcriptional differences between mouse ES cells from the D3 cell line and transformed beta-cells (MIN6 cell line) as determined by a direct comparative analysis of their transcriptome. The results show that 40% of transcripts were differentially expressed between D3 and MIN6 cells. There is thus a marked difference in the pattern of transcription between the ES cell and beta-cell genomes. Total RNA was extracted from three independent replicates of passage 21 D3 cells and passage 23 MIN6 cells. A whole mouse genome comparison between D3 and MIN6 cells was performed using the Mouse WG-6 v2.0 Expression Beadchip from Illumina.
Experiment type
transcription profiling by array 
Contacts
Chris O'Neill <chris.oneill@sydney.edu.au>, Christopher O'Neill, Jennifer Y Wong, Michelle M Jack
MIAME
PlatformsProtocolsFactorsProcessedRaw
Files
Investigation descriptionE-GEOD-49077.idf.txt
Sample and data relationshipE-GEOD-49077.sdrf.txt
Processed data (1)E-GEOD-49077.processed.1.zip
Array designA-MEXP-1175.adf.txt
Links