E-GEOD-49069 - The role of Dichaete in transcriptional regulation of Drosophila embryogenesis

Status
Released on 22 July 2013, last updated on 3 June 2014
Organism
Drosophila melanogaster
Samples (8)
Array (1)
Protocols (7)
Description
Dichaete is a developmentally important transcription factor, known to be involved in basic biological processes including segmentation and nervous system development among others. The aim of this experiment was to gain further insight into the role of Dichaete during early embryogenesis, by looking at the disruption of gene expression in Dichaete mutants. Stage 10-11 embryos (5 and 7.5 hours after egg laying) from a cross between Dr72/TM3, twi-GAL4 UAS-Gfp Dr513/TM3, twi-GAL4 UAS-Gfp, were hand picked under a fluorescence dissecting microscope. GFP negative homozygous Dichaete mutant embryos and their heterozygous single GFP positive siblings were collected and approximately 150 embryos per sample were stored frozen in Trizol
Experiment type
transcription profiling by array 
Contacts
Steve Russell <sr120@hermes.cam.ac.uk>, Bettina Fischer, Jelena Aleksic
MIAME
PlatformsProtocolsFactorsProcessedRaw
Files
Investigation descriptionE-GEOD-49069.idf.txt
Sample and data relationshipE-GEOD-49069.sdrf.txt
Raw data (1)E-GEOD-49069.raw.1.zip
Processed data (1)E-GEOD-49069.processed.1.zip
Array designA-GEOD-14121.adf.txt
Links