E-GEOD-49037 - WRKY6 Transcription Factor Restricts Arsenate Uptake and Transposon Activation in Arabidopsis
Released on 25 April 2014, last updated on 6 May 2014
Stress constantly challenges plant adaptation to the environment. Of all stress types, arsenic was a major threat during the early evolution of plants. The most prevalent chemical form of arsenic is arsenate, whose similarity to phosphate renders it easily incorporated into cells via the phosphate transporters. Here we found that arsenate stress provokes a notable transposon burst in plants, in coordination with arsenate/phosphate transporter repression, which immediately restricts arsenate uptake. This repression was accompanied by delocalization of the phosphate transporter from the plasma membrane. When arsenate was removed, the system rapidly restored transcriptional expression and membrane localization of the transporter. We identify WRKY6 as an arsenate-responsive transcription factor that mediates arsenate/phosphate transporter gene expression and restricts arsenate-induced transposon activation. Plants therefore have a dual WRKY-dependent signaling mechanism that modulates arsenate uptake and transposon expression, providing a coordinated strategy for arsenate tolerance and transposon gene silencing. Three biological replicates were performed for each sample type. Single channel hybridizations were carried-out using either Affymetrix ATH1 platform or Nimblegen Gene Expression 12x135K platform (Arabidopsis thaliana).
transcription profiling by array
Juan Carlos Oliveros <firstname.lastname@example.org>, Alfonso Mouriz, Ana Fraile-Escanciano, Antonio Leyva, Eduardo Sánchez-Bermejo, Enrique Rojo, Gabriel Castrillo, Isabel Mateos, Javier Paz-Ares, Jose A Jarillo, Juan Sobrino-Plata, Laura de Lorenzo, Luis E Hernández, Manuel Piñeiro, Mohan TC, Pablo Catarecha, Pedro Crevillen, Sanna Olsson, Yolanda Leo del Puerto
WRKY6 transcription factor restricts arsenate uptake and transposon activation in Arabidopsis. Castrillo G, Sï¿½nchez-Bermejo E, de Lorenzo L, Crevillï¿½n P, Fraile-Escanciano A, Tc M, Mouriz A, Catarecha P, Sobrino-Plata J, Olsson S, Leo Del Puerto Y, Mateos I, Rojo E, Hernï¿½ndez LE, Jarillo JA, Piï¿½eiro M, Paz-Ares J, Leyva A. , Europe PMC 23922208