E-GEOD-49030 - Genome-wide profiling of the activity-dependent hippocampal transcriptome
Released on 23 October 2013, last updated on 3 June 2014
Activity-dependent gene expression is central for sculpting neuronal connectivity in the brain. Despite the importance for synaptic plasticity, a comprehensive analysis of the temporal changes in the transcriptomic response to neuronal activity is lacking. In a genome wide survey we identified genes that were induced at 1, 4, 8, or 24 hours following neuronal activity in the hippocampus. 3 month old male mice were injected with kainic acid or isotonic saline solution. Animals were sacrificed by cervical luxation 1, 2, 4, 8, or 24 h after onset of the first seizure, and RNA was extracted from the hippocampal region.
transcription profiling by array
Nils Blüthgen <firstname.lastname@example.org>, Claudia Mahlke, Dietmar Kuhl, Guido Hermey, Jakob J Gutzmann, Jörg Schreiber
Genome-wide profiling of the activity-dependent hippocampal transcriptome. Hermey G, Mahlke C, Gutzmann JJ, Schreiber J, Blï¿½thgen N, Kuhl D. , Europe PMC 24146943