E-GEOD-49019 - HIV-1 gp120 impairs B cell proliferation by inducing TGF-β1 production and FcRL4 expression via an α4β7-dependent mechanism

Status
Released on 17 October 2013, last updated on 28 October 2013
Organism
Homo sapiens
Samples (42)
Array (1)
Protocols (7)
Description
The anti-HIV humoral immune response following acute infection is delayed and ineffective. HIV envelope protein gp120 binds to and signals through α4β7 on T cells. We show that gp120 also binds and signals through α4β7 on B cells, resulting in an abortive proliferative response. In primary B cells, gp120 signaling through α4β7 resulted in increased expression of TGF-β1 and the B cell inhibitory receptor FcRL4. Co-culture of B cells with HIV-infected autologous CD4+ T cells also resulted in increased B cell FcRL4 expression. These findings indicate that, in addition to inducing chronic immune activation, viral proteins can contribute directly to HIV-associated B cell dysfunction, thus providing a mechanism whereby the virus subverts the early HIV-specific humoral immune response. Forty-two samples in two batches were run with different treatments and different time points.
Experiment type
transcription profiling by array 
Contacts
Richard Lempicki <geo@ncbi.nlm.nih.gov>, Anthony Fauci, Antonio David, Catherine Schwing, Claudia Cicala, Da W Huang, Danlan Wei, Don Van Ryk, Fatima Nawaz, Greg Roby, II Y Hwang, James Arthos, Joe Hiatt, John H Kehrl, Jun Yang, Katija Jelicic, Massimiliano Pascuccio, Raffaello Cimbro, Xin Zheng
MIAME
PlatformsProtocolsFactorsProcessedRaw
Files
Investigation descriptionE-GEOD-49019.idf.txt
Sample and data relationshipE-GEOD-49019.sdrf.txt
Raw data (1)E-GEOD-49019.raw.1.zip
Processed data (1)E-GEOD-49019.processed.1.zip
Array designA-AFFY-141.adf.txt
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