E-GEOD-49003 - Gene expression analysis of metastatic and non-metastatic osteosarcoma cell lines

Status
Released on 19 July 2013, last updated on 3 June 2014
Organism
Homo sapiens
Samples (12)
Array (1)
Protocols (18)
Description
Analysis of two metastatic OS cell lines, KHOS and KRIB, and two non-metastatic OS cell lines, HOS and U2OS. Results show differences in gene expression between cell lines with different ability to metastasise in vivo. Each of the four cell lines were analyzed in tripicate. Total RNA was extracted from each triplicate cell line culture with Trizol. cRNA was amplified using the Ambion Illumina Total Prep RNA Amplification Kit. Concentration was determined in NanoDrop and qulity checked in the Agilent Bioanalyzer, before hybridizing to Illumina HT-12 BeadChip Array.
Experiment type
transcription profiling by array 
Contacts
Liliana Beatriz Endo-Munoz <l.munoz@uq.edu.au>, Liliana Endo-Munoz, Nicholas A Saunders
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-49003.idf.txt
Sample and data relationshipE-GEOD-49003.sdrf.txt
Processed data (1)E-GEOD-49003.processed.1.zip
Array designA-MEXP-1171.adf.txt
Links